Screening for Polyphenol Oxidase in Enologically Important Yeast Cultures
Abstract: Polyphenol oxidases area unit enzymes that turn the oxidization of phenol compounds exploitation molecular atomic number 8. the power of polyphenol oxidases to act on synthetic resin compounds makes them extremely helpful biocatalysts for numerous biotechnological applications. they're unremarkably found in animals, plants and fungi. Recent ordination analysis have shown that polyphenol oxidases also are widespread in yeast species. within the gift study, baker's yeast (ITB), fungus genus (S101), Pichia kudriavzeii (P1), AP[C] and Strain C were screened and characterize polyphenol enzyme (PPO) for his or her hydrogen ion concentration, temperature, accelerator kinetic, substrate specificity.
More hand-picked organisms were screened for hydrogen ion concentration and thermal stability. Spectrophotometric methodology was accustomed assay the PPO activity, by activity the initial rate of benzoquinone formation, as indicated by a rise in absorbance. Initial results of activity measurements of samples from animate thing and living thing extract discovered that the accelerator was animate thing. more work was dispensed with animate thing enzymes. results of screening discovered the P1 to supply one.9 g/ 100ml of dry biomass & the accelerator etxracted from Strain C and P1, exhibits the most activity at optimum hydrogen ion concentration five & seven and 20°C & 25°C.
The optimum temperature achieved for accelerator production was 25oC for each organisms. Characterization studies indicated that the accelerator showed highest activity at hydrogen ion concentration vi.0 and 30ºC. klick and Vmax values for the accelerator were determined as zero.21 & 0.17 metric linear unit for Strain C and P1, severally. As for the thermal stability studies, the PPO activity belittled with increasing temperature.
Denaturation of this accelerator was measured by loss in activity. Efforts to spot strain C exploitation morphological characters (SEM), FTIR studies and 18sR DNA were dispensed. FTIR analysis indicates that the unidentified strain resembles Pichia sp. more confirmation of molecular analysis is hoped-for.
Author: Swati, Dahariya